Molecular study of selected taxonomically critical taxa of the genus Iris L. from the broader Alpine-Dinaric area

Weber, Tim (avtor), Jakše, Jernej (avtor), Sladonja, Barbara (avtor), Hruševar, Dario (avtor), Landeka, Nediljko (avtor), Brana, Slavko (avtor), Bohanec, Borut (avtor), Milović, Milenko (avtor), Vladović, Dalibor (avtor), Mitić, Božena (avtor), Poljuha, Danijela (avtor)

Some wild, morphologically diverse taxa of the genus Iris in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical Iris taxa from that area (especially a narrow endemic accepted species I. adriatica, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (ndhJ, rpoC1) markers to the understanding of the Iris taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid markers-based maximum likelihood analysis discriminated three main clusters in the set of 32 analysed samples, which correspond well to the lower taxonomic categories of the genus, and support separate status of ambiguous regional taxa (e.g., I. sibirica subsp. erirrhiza, I. x croatica and I. x rotschildii). The first molecular data on I. adriatica revealed its genome size (2C = 12.639 ± 0.202 pg) and indicated the existence of ecotypes. For future molecular characterisation of the genus we recommend the utilisation of microsatellite markers supplemented with a combination of plastid markers.

• Jezik: Angleški jezik
• Ključne besede: Iridaceae, Europe, chloroplast DNA, microsatellites, phylogeny, taxonomy
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 16 str.
• Številčenje: Vol. 9, iss. 9, art. 1229
• PID: 20.500.12556/RUL-134235
• UDK: 577.2
• ISSN pri članku: 2223-7747
• DOI: 10.3390/plants9091229
• COBISS.SI-ID: 29832451
• Datum objave v RUL: 03.01.2022

Gradivo je del revije

• Naslov: Plants
• Skrajšan naslov: Plants
• Založnik: MDPI
• ISSN: 2223-7747
• COBISS.SI-ID: 523345433

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 18.09.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Iridaceae, mikrosatelitski markerji, filogenija, molekularna biologija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0077
• Naslov: Kmetijske rastline - genetika in sodobne tehnologije

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: University of Zagreb
• Številka projekta: 106-F20-00025