Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(lactide-co-glycolide) after subchronic oral exposure of rats

Paraš, Smiljana (avtor), Trišić, Dijana (avtor), Mitrović Ajtić, Olivera (avtor), Prokić, Bogomir (avtor), Drobne, Damjana (avtor), Živković, Slavoljub (avtor), Jokanović, Vukoman (avtor)

Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.

• Jezik: Angleški jezik
• Ključne besede: hydroxyapatite, bone substitute, genotoxicity, subchronic toxicity, biocompatibility
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 21 str.
• Številčenje: Vol. 10, iss. 5, art. 918
• PID: 20.500.12556/RUL-133739
• UDK: 577
• ISSN pri članku: 2079-4991
• DOI: 10.3390/nano10050918
• COBISS.SI-ID: 15090691
• Datum objave v RUL: 13.12.2021

Gradivo je del revije

• Naslov: Nanomaterials
• Skrajšan naslov: Nanomaterials
• Založnik: MDPI
• ISSN: 2079-4991
• COBISS.SI-ID: 523286297

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 09.05.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: biokemija, genotoksičnost, kostni nadomestki, nanomateriali

Projekti

• Financer: MESTD - Ministry of Education, Science and Technological Development of Republic of Serbia
• Program financ.: Basic Research (BR or ON)
• Številka projekta: 172026
• Naslov: Chemical and structural designing of nanomaterials for application in medicine and tissue engineering

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J1-9162
• Naslov: Neurotoksičnost ali neuroprotektivnost nanomaterialov: vpliv biokorone