Viscosity of plasma as a key factor in assessment of extracellular vesicles by light scattering

Božič, Darja (avtor), Sitar, Simona (avtor), Junkar, Ita (avtor), Štukelj, Roman (avtor), Pajnič, Manca (avtor), Žagar, Ema (avtor), Kralj-Iglič, Veronika (avtor), Kogej, Ksenija (avtor)

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.

• Jezik: Angleški jezik
• Ključne besede: extracellular vesicles, exosomes, blood plasma, dynamic light scattering, static light scattering, shape characterization, viscosity of blood plasma
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo ; ZF - Zdravstvena fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: 21 str.
• Številčenje: Vol. 8, iss. 9, art. 1046
• PID: 20.500.12556/RUL-132807
• UDK: 577.35
• ISSN pri članku: 2073-4409
• DOI: 10.3390/cells8091046
• COBISS.SI-ID: 1538312131
• Datum objave v RUL: 03.11.2021

Gradivo je del revije

• Naslov: Cells
• Skrajšan naslov: Cells
• Založnik: MDPI
• ISSN: 2073-4409
• COBISS.SI-ID: 519958809

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 06.09.2019

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: izvencelični vezikli, eksosomi, krvna plazma, dinamično sipanje svetlobe, statično sipanje svetlobe, določanje oblike, viskoznost krvne plazme