The Escherichia coli colibactin resistance protein ClbS is a novel DNA binding protein that protects DNA from nucleolytic degradation

Molan, Katja (avtor), Podlesek, Zdravko (avtor), Hodnik, Vesna (avtor), Butala, Matej (avtor), Oswald, Eric (avtor), Žgur-Bertok, Darja (avtor)

Cells employ specific and nonspecific mechanisms to protect their genome integrity against exogenous and endogenous factors. The clbS gene is part of the polyketide synthase machinery (pks genomic island) encoding colibactin, a genotoxin implicated in promoting colorectal cancer. The pks is found among the Enterobacteriaceae, in particular Escherichia coli strains of the B2 phylogenetic group. Several resistance mechanisms protect toxin producers against toxicity of their products. ClbS, a cyclopropane hydrolase, was shown to confer colibactin resistance by opening its electrophilic cyclopropane ring. Here we report that ClbS sustained viability and enabled growth also of E. coli expressing another genotoxin, the Usp nuclease. The recA::gfp reporter system showed that ClbS protects against Usp induced DNA damage. To elucidate the mechanism of ClbS mediated protection, we studied the DNA binding ability of the ClbS protein. We show that ClbS directly interacts with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), whereas ssDNA seems to be the preferred substrate. Thus, the ClbS DNA-binding characteristics may serve bacteria to protect their genomes against DNA degradation.

• Jezik: Angleški jezik
• Ključne besede: ClbS, DNA-binding protein, DNA damage protection, pks genomic island, Escherichia coli
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: Str. 50-54
• Številčenje: Vol. 79
• PID: 20.500.12556/RUL-128248
• UDK: 579
• ISSN pri članku: 1568-7856
• DOI: 10.1016/j.dnarep.2019.05.003
• COBISS.SI-ID: 5079119
• Datum objave v RUL: 07.07.2021

Gradivo je del revije

• Naslov: DNA Repair
• Založnik: Elsevier
• ISSN: 1568-7856
• COBISS.SI-ID: 24102405

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.07.2019