The first detection and genetic characterization of four different honeybee viruses in wild bumblebees from Croatia

Tlak Gajger, Ivana (avtor), Šimenc Kramar, Laura (avtor), Toplak, Ivan (avtor)

To determine the presence and the prevalence of four different honeybee viruses (acute bee paralysis virus—ABPV, black queen cell virus—BQCV, chronic bee paralysis virus—CBPV, deformed wing virus—DWV) in wild bumblebees, pooled randomly selected bumblebee samples were collected from twenty-seven different locations in the territory of Croatia. All samples were prepared and examined using the RT-PCR methods for quantification of mentioned honeybee viruses. Determined prevalence (%) of identified positive viruses were in the following decreasing order: BQCV > DWV > ABPV, CBPV. Additionally, direct sequencing of samples positive for BQCV (n = 24) and DWV (n = 2) was performed, as well as a test of molecular phylogeny comparison with those available in GenBank. Selected positive field viruses’ strains showed 95.7 to 100% (BQCV) and 98.09% (DWV) nucleotide identity with previously detected and deposited honeybee virus strains in the geographic areas in Croatia and neighboring Slovenia. In this article, the first detection of four honeybee viruses with genetic characterization of high diversity strains circulating in wild bumblebees in Croatia is presented.

• Jezik: Angleški jezik
• Ključne besede: Bombus spp., honeybee viruses, deformed wing virus, black queen cell virus, acute bee paralysis virus, chronic bee paralysis virus, genetic characterization, sequencing, transmission routes, viruses, genetics, veterinary epidemiology, bees, virology, bumblebees
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 11 str.
• Številčenje: Vol. 10, iss. 7, art. 808
• PID: 20.500.12556/RUL-128032
• UDK: 638:578
• ISSN pri članku: 2076-0817
• DOI: 10.3390/pathogens10070808
• COBISS.SI-ID: 68557315
• Datum objave v RUL: 01.07.2021

TLAK GAJGER, Ivana, ŠIMENC KRAMAR, Laura in TOPLAK, Ivan, 2021, The first detection and genetic characterization of four different honeybee viruses in wild bumblebees from Croatia. Pathogens [na spletu]. 2021. Vol. 10, no. 7,  808. [Dostopano 5 maj 2025]. DOI 10.3390/pathogens10070808. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=128032

Gradivo je del revije

• Naslov: Pathogens
• Skrajšan naslov: Pathogens
• Založnik: MDPI
• ISSN: 2076-0817
• COBISS.SI-ID: 523338009

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.07.2021