A rapid analysis of bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals

Cerkvenik, Vesna (avtor), Šturm, Sabina (avtor)

Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 μg/kg were in the range of 67–86% and 3–34%, respectively, while linearity in the matrix, represented by the r$^2$, was ≥0.999. LOD values were 0.5–1 μg/kg and LOQ values were 1 μg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics. •The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection. •MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values. •It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision.

• Jezik: Angleški jezik
• Ključne besede: bisphenol A, tissue analysis, solid phase extraction, molecularly imprinted polymers, liquid chromatography, bisphenol A-glycidyl methacrylate
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 14 str.
• Številčenje: Vol. 8, art. 101351
• PID: 20.500.12556/RUL-127091
• UDK: 615.9
• ISSN pri članku: 2215-0161
• DOI: 10.1016/j.mex.2021.101351
• COBISS.SI-ID: 62349827
• Datum objave v RUL: 17.05.2021

Gradivo je del revije

• Naslov: MethodsX
• Založnik: Elsevier
• ISSN: 2215-0161
• COBISS.SI-ID: 13645083

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana