Copolymeric hydrogel-based immobilization of yeast cells for continuous biotransformation of fumaric acid in a microreactor

Menegatti, Tadej (avtor), Žnidaršič Plazl, Polona (avtor)

Although enzymatic microbioreactors have recently gained lots of attention, reports on the use of whole cells as biocatalysts in microreactors have been rather modest. In this work, an efficient microreactor with permeabilized Saccharomyces cerevisiae cells was developed and used for continuous biotransformation of fumaric into industrially relevant L-malic acid. The immobilization of yeast cells was achieved by entrapment in a porous structure of various hydrogels. Copolymers based on different ratios of sodium alginate (SA) and polyvinyl alcohol (PVA) were used for hydrogel formation, while calcium chloride and boric or phenylboronic acid were tested as crosslinking agents for SA and PVA, respectively. The influence of hydrogel composition on physico-chemical properties of hydrogels prepared in the form of thin films was evaluated. Immobilization of permeabilized S. cerevisiae cells in the selected copolymeric hydrogel resulted in up to 72% retained fumarase activity. The continuous biotransformation process using two layers of hydrogels integrated into a two-plate microreactor revealed high space time yield of 2.86 g/(L·h) while no activity loss was recorded during 7 days of continuous operation.

• Jezik: Angleški jezik
• Ključne besede: microreactor, copolymeric hydrogel, immobilization, biotransformation, L-malic acid, whole-cell biocatalysis, Saccharomyces cerevisiae, microbioreactor
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: 12 str.
• Številčenje: Vol. 10, iss. 12, art. 867
• PID: 20.500.12556/RUL-127084
• UDK: 66.098
• ISSN pri članku: 2072-666X
• DOI: 10.3390/mi10120867
• COBISS.SI-ID: 1538481859
• Datum objave v RUL: 17.05.2021

Gradivo je del revije

• Naslov: Micromachines
• Skrajšan naslov: Micromachines
• Založnik: Multidisciplinary Digital Publishing Institute (MDPI)
• ISSN: 2072-666X
• COBISS.SI-ID: 523276825

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 10.12.2019

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikroreaktorji, kopolimerni hidrogeli, imobilizacija, biotransformacije, L-jabolčna kislina, celična biokataliza, Saccharomyces cerevisiae