A genetically modfied organism, with the exception of a human being, is an organism or micro-organism whose genetic material is altered by processes that alter the genetic material differently than it occurs in nature. They can be potentially dangerous, toxic, or have the potential for uncontrolled spreading. There are four possible biosafety levels into which we classify them. Each level determines a combination of different measures, guidelines, layout of laboratory space, safety and other laboratory equipment that must be satisfied in order for work safely in a laboratory for genetically modfied organisms. Biosafety level II, which is the focus of our interest, includes genetically modfied organisms that pose little risk to humans and the environment. The purpose of the master's thesis was to plan strategies to establish good laboratory practice for working with biosafety level II genetically modfied organisms in vitro. We have came to conclusion that, in order to start such laboratory in the Republic of Slovenia, it is necessary to follow the conditions regulated by the law and legislation from this field. The conditions are as follows: the working surfaces of the laboratory must be easy to clean and resistant to water, acids, bases, solvents, disinfectabts and decontaminants. There must be an autoclave in the building where the laboratory is located. Access to the closed system must be restricted, with a biohazard sign on the door. It is mandatory to establish special measures to control the spread of aerosols. Protective clothing is required. It is mandatory to prevent contact of vectors such as insects and rodents with laboratory. Inactivation of genetically modfied organisms is mandatory in all materials and wastes that are genetically modfied organisms or have come into contact with them. Since the layout of the equipment inside the laboratory space is also responsible for the smooth operation of the laboratory system itself, we decided to include that part in this master's thesis. We decided to develop various layouts of laboratory equipment in the room available for the laboratory for work with biosafety level II genetically modfied organisms in the Faculty of pharmacy in Ljubljana. We compared the instructions of various authors in the field of arranging cell laboratories, and finally made our own floor plan. Among other things, we found how the implementation of good microbiological practice contributes to the prevention of contamination within the laboratory. Good microbiological practice complements containment measures, procedures and processes designed to maintain safety both inside and outside of the laboratory.
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