Methylation of selenocysteine catalysed by thiopurine S-methyltransferase

Urbančič, Dunja (avtor), Kotar, Anita (avtor), Šmid, Alenka (avtor), Jukič, Marko (avtor), Gobec, Stanislav (avtor), Martensson, Lars (avtor), Plavec, Janez (avtor), Mlinarič-Raščan, Irena (avtor)

Background: Methylation driven by thiopurine S-methylatransferase (TPMT) is crucial for deactivation of cytostatic and immunosuppressant thiopurines. Despite its remarkable integration into clinical practice, the endogenous function of TPMT is unknown. Methods: To address the role of TPMT in methylation of selenium compounds, we established the research on saturation transfer difference (STD) and 77Se NMR spectroscopy, fluorescence measurements, as well as computational molecular docking simulations. Results: Using STD NMR spectroscopy and fluorescence measurements of tryptophan residues in TPMT, we determined the binding of selenocysteine (Sec) to human recombinant TPMT. By comparing binding characteristics of Sec in the absence and in the presence of methyl donor, we confirmed S-adenosylmethionine (SAM)-induced conformational changes in TPMT. Molecular docking analysis positioned Sec into the active site of TPMT with orientation relevant for methylation reaction. Se-methylselenocysteine (MeSec), produced in the enzymatic reaction, was detected by 77Se NMR spectroscopy. A direct interaction between Sec and SAM in the active site of rTPMT and the formation of both products, MeSec and S-adenosylhomocysteine, was demonstrated using NMR spectroscopy. Conclusions: The present study provides evidence on in vitro methylation of Sec by rTPMT in a SAM-dependant manner. General significance: Our results suggest novel role of TPMT and demonstrate new insights into enzymatic modifications of the 21st amino acid.

• Jezik: Angleški jezik
• Ključne besede: binding analysis, enzymatic methylation, NMR spectroscopy, S-adenosylmethionine, selenocysteine, thiopurine S-methyltransferase
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2019
• Št. strani: Str. 182-190
• Številčenje: Vol. 1863, iss. 1
• PID: 20.500.12556/RUL-125358
• UDK: 543.429.23:615
• ISSN pri članku: 0304-4165
• DOI: 10.1016/j.bbagen.2018.10.002
• COBISS.SI-ID: 4616561
• Datum objave v RUL: 12.03.2021

URBANČIČ, Dunja, KOTAR, Anita, ŠMID, Alenka, JUKIČ, Marko, GOBEC, Stanislav, MARTENSSON, Lars, PLAVEC, Janez in MLINARIČ-RAŠČAN, Irena, 2019, Methylation of selenocysteine catalysed by thiopurine S-methyltransferase. Biochimica et biophysica acta (G). General subjects [na spletu]. 2019. Vol. 1863, no. 1, p. 182–190. [Dostopano 11 april 2025]. DOI 10.1016/j.bbagen.2018.10.002. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=125358

Gradivo je del revije

• Naslov: Biochimica et biophysica acta (G). General subjects
• Skrajšan naslov: Biochim. biophys. acta (G)
• Založnik: Elsevier
• ISSN: 0304-4165
• COBISS.SI-ID: 20760839

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
• Začetek licenciranja: 12.03.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: jedrska magnetna resonanca, analizne metode, S-metiltransferaze