Goal of our thesis was to study the effect of different treatments before germination of spelt grains on mold population, and germinability and growth intensity of treated splet grains. As treatments we used hot water (5 and 10 minutes of treatment at 50 °C), hydrogen peroxide (10 % and 20 % concentration) and sodium hypochlorite (0,5 % and 1 % concentration). We treated soaked and non-soaked grain. Due to low natural contamination of spelt grains with molds, we contaminated samples with Penicillium griseofulvum. After treatments we proceed with mold quantification, germinability of seeds and measurments of acrospire and rootlets. The results were presented as average of all measurments. The treatments achieved different percentages of inhibiton of P. griseofulvum. We confirmed the greatest inhibition of P. griseofulvum at treatments with 1 % sodium hypochlorite (96,7 %) and 20 % hydrogen peroxide (96,2 %). We obsereved very good germinability (over 90 %) in all treatments of both soaked and non-soaked grain after 72 hours. We can conclude, that different treatments did not lower the seed germinability. Lenght measuments of acrospire and rootlets showed great variations between different treatments. We confirmed that, mainly with greater contentration of hydrogen peroxide and sodium hypochlorite there is a lower intensity of acrospire and rootlet growth. We can conclude, that there could be more promising research in that area with different combinations of time and temperature or even combinations of said treatments. Because we didn't use agressive compunds, and grain treatments are usually done before germination, the future research of non-soaked grains with hot water treatment promises practical results.
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