The goal of the thesis was to study the chemical structure of Abies alba Mill's bark and to identify the antioxidative and fungicide potential of gathered extracts. At first, we needed to collect samples of trunks bark every two weeks. Absolutely dry samples were ground and placed in reagent bottles. Extraction was executed using an accelerated solvent extractor. Then we preformed chemical analysis of the extracts (gravimetric analysis, UV-Vis spectrophotometry, TLC and HPLC) and the DPPH method was used to determine the antioxidative potential. Fungicide potential was divided into three mould and three fungus test samples. We discovered that humidity decreases with time and that the bark includes more hydrophilic extractives. On average the outer layer contains more hydrophilic extractives, phenols, catechin and epicatechin than the inner layer. On average the tree bark samples that were taken higher from the tree contain more hydrophilic extractives. Branches growing lower on the trunk contain more hydrophilic extractives and phenols in their bark. With TLC we determined that our sample contained 14 compounds. Through HPLC analysis, we found that our extracts contained the most catechin, epicatechin and taxifolin. The extract from the bark of branches has the greatest antioxidative potential. The higher the concentration of the extract, the greater the inhibitory effect on the growth of fungi and mould it has. The extract obtained from the bark of the branches has a greater inhibitory effect than the extract obtained from the bark of the trunk.
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