Honey is a natural sweet substance, which is produced by honey bees from nectar or honeydew by the addition of their own secretions. Due to the increased bacterial resistance to antibiotics and the increasing knowledge of honey's ingredients and their interactions, the potential of honey in the fight against bacterial-borne diseases is increasing. Since honey's antimicrobial and antiadhesive activity is well known, it has been assumed that honey will act as inhibitor in biofilm formation in oral cavity, thereby preventing attachment and spread of pathogenic bacteria and related diseases of oral cavity. We studied the impact of chestnut honey on Streptococcus cristatus adhesion on dental surface. We have optimized method for quantification of bacterial adhesion on dental surface (cleaning of tooth surfaces prior adhesion, the way of dental surface exposure to bacteria). Using the optimized method, we found that chestnut honey had no effect on Strept. cristatus adhesion to dental surface. In order to further characterize the antiadhesive activity of the honey on dental surface, several concentrations of chestnut honey should be tested, the chemical properties of chestnut honey should be analysed, dental surfaces should be evaluated and a greater number of them should be used in all analyses.
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