The purpose of the present experiment was to determine the effect of two extenders and two temperatures of thawing on fertilizing ability and quality of fresh and cryopreserved rooster semen. Semen was collected from twenty-eight roosters and 135 hens of the same layer-type strain were used for fertility trails. All birds were 71 weeks old. Hens were randomly divided into 5 groups. One of these groups served as a control group. Semen samples were collected with abdominal massage, pooled together and divided into two parts. One part was diluted with basic extender HS1 and the other part was diluted with extender HS1, which contained 20% extract of aloe vera. The stored semen was thawed at 5°C and 75°C before being used for insemination or laboratory analyses. Sperm viability, motility and progressive motility were assessed in fresh and frozen-thawed semen which were also used for artificial insemination. After incubation period of 21 days embryonic deaths, infertile eggs and hatchability rates were determined separately by experimental groups. Extender HS1 significantly (p<0,0001) improved egg fertility and hatchability in comparison with extender which contained aloe vera. Fertility and hatchability were not affected by temperature of thawing. Viability of sperm cells was significantly (p<0,05) higher in extender HS1 in comparison with extender which contained aloe vera and significantly (p<0,05) higher when temperature of thawing was 5°C in comparison with 75°C. Total motility and progressive motility were significantly (p<0,05) higher in extender HS1 in comparison with extender which contained aloe vera, while temperature of thawing did not affect the motility of sperm cells.
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