The aim of this thesis was to compare the oxidative state in cells of wild type and the highproducer strain of bacterium Saccharopolyspora erythraea during the erythromycin production and also to establish potencial connection between oxidative stress and secondary metabolism. We set up bioprocess with wild type and the industrial high-producing strain, where parameters like pH, partial mycelium volume, glucose and erythromycin concentration were measured. To evaluate oxidative state, we used 2',7'-dichlorodihydrofluorescein to measure intracellular oxidation. Intracellular oxidation level was higher during the intensive production of erythromycin in the industrial strain, while in the wild type strain there were no noticeable changes. Furthermore, we determined the catalase activity by measuring the decrease in absorbance of sample during hydrogen peroxide degradation. We have not observed any changes in catalase activity, independently on the strain used. Although we observed higher intracellular oxidation during intensive erythromycin production in the high-producing strain, catalase activity was still comparable with the wild type strain. Our results indicate, that there is a positive correlation between oxidative stress and erythromycin production in Saccharopolyspora erythraea.
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